25 tests / Grape Must pack
Items provided: 25 Botrytis prototype test strips, with 25 buffer bottles, 25 bulb sample transfer pipettes, and 25 sample cups.
Instructions for Use: These are provided in packs, and can be downloaded from BotrytisAlert website.
Storage and Shelf Life: Store in original packaging between 15-30 °C, out of direct sunlight, in dry conditions.
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ISCLAIMER
The purpose of this diagnostic device is to detect botrytis mycelium in plant material or in air samples. The test uses monoclonal BC-12.CA4 which is described as a botrytis-specific monoclonal antibody (Meyer and Dewey, 2000 & Meyer & Spotts, 2000) that has been found useful in the detection and quantification of B. cinerea in juice from grapes for wine production and plant material (Dewey, F. M., Ebeler, S. E., Adams, D. O., Noble, A. C., and Meyer, U. M., . J. Enol. Vitic. & Meyer & Spotts, 2000).
BC-12.CA4 is reported to recognize an antigen, possibly a glycoprotein, with the antigenic binding site on L-rhamnose. The antigen is expressed from the first appearance of the germ tube during germination and can be observed by immunofluorescence along the entire length and tip of the germ tube but not on conidia.
The test does not discriminate viable and non-viable botrytis infection state. So, should not be used to assess the efficacy of control treatment. For example, a control treatment may be applied and kill the botrytis. However the botrytis antigen will likely remain and be detected by the test.
The test is a preliminary screen and it is recommended that results be confirmed by an alternate method.
The test result will depend on the sample assessed, the sample size and the sampling process. A negative result does not preclude the presence of botrytis.
As the test may well be used for field & protected crop testing activities protect the test during storage by avoiding hot or cold temperature extremes. Do not leave in direct sunlight or in a vehicle.
Mologic does not give any representations or warranties, whether express or implied, including but not limited to warranties or merchantability, fitness for a particular purpose, or title, or of non-infringement of third party rights.
Use of the Diagnostic Device is at the users risk and Mologic does not assume any liability whatsoever arising out of use of the Diagnostic Device
References:
Meyer, U. M., and Dewey, F. M. 2000. Efficacy of different immunogens for raising monoclonal antibodies to botrytis cinerea. Mycol. Res. 104 (8): pp 979-987.doi:10.1017/S0953756200002501.
Meyer, U.M., Spotts, R.A and Dewey, F.M. 2000. Detection and quantification of botrytis cinerea by ELISA in pear stems during cold storage. Plant Disease, 84:1099-1103.https://apsjournals.apsnet.org/doi/pdf/10.1094/PDIS.2000.84.10.1099
Dewey, F. M., Ebeler, S. E., Adams, D. O., Noble, A. C., and Meyer, U. M. 2000. Quantification of botrytis in grape juice determined by a monoclonal antibody-based immunoassay. Am. J. Enol. Vitic. 51:276-282. https://www.ajevonline.org/content/51/3/276